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KMID : 0811720080120020059
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 2 p.59 ~ p.64
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Relaxant Effect of Spermidine on Acethylcholine and High K+- induced Gastric Contractions of Guinea-Pig
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Kim Young-Chul
Sim Jae-Hoon
Kim Chan-Hyung
Choi Woong
Lee Sang-Jin
Xu Wen-Xie
You Ra-Young
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Abstract
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In our previous study, we found that spermine and putrescine inhibited spontaneous and acetylcholine (ACh)-induced contractions of guinea-pig stomach via inhibition of L-type voltage- dependent calcium current (VDCCL). In this study, we also studied the effect of spermidine on mechanical contractions and calcium channel current (IBa), and then compared its effects to those by spermine and putrescine. Spermidine inhibited spontaneous contraction of the gastric smooth muscle in a concentration-dependent manner (IC50=1.1±0.11 mM). Relationship between inhibition of contraction
and calcium current by spermidine was studied using 50 mM high K+-induced contraction: Spermidine (5 mM) significantly reduced high K+ (50 mM)-induced contraction to 37±4.7% of the control (p＜ 0.05), and inhibitory effect of spermidine on IBa was also observed at a wide range of test potential in current/voltage (I/ V) relationship. Pre- and post-application of spermidine (5 mM) also significantly inhibited carbachol (CCh) and ACh-induced initial and phasic contractions. Finally, caffeine (10 mM)-induced contraction which is activated by Ca2+-induced Ca2+ release (CICR),` was also inhibited by pretreatment of spermidine (5 mM). These findings suggest that spermidine inhibits spontaneous
and CCh-induced contraction via inhibition of VDCCL and Ca2+ releasing mechanism in guinea-pig stomach.
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KEYWORD
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Stomach, Relaxation, Calcium current, Ca2+ release, Spermidine
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